A PCR assay to quantify patterns of HBV transcription
نویسندگان
چکیده
منابع مشابه
A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype
Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...
متن کاملPCR-Based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells.
An assay that quantifies the amount of human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood mononuclear cells has been developed. PCR amplification of the HIV-1 DNA is performed in the presence of an internal quantitation standard, and colorimetric detection of the amplified product is performed with microwell plates. The copies of HIV-1 DNA are normalized to total genomic DNA in...
متن کاملDetection of HBV DNA in serum using a PCR-based assay.
HBV DNA in viral particles in serum is covered by a coat of hepatitis B core antigen (HBcAg) particles and a lipid coat with hepatitis B surface antigen (HBsAg) in it. Removal of the HBcAg and the HBsAg with the lipid coat can be easily accomplished by treatment with a detergent or alkali. However, there are many inhibitors of the PCR reaction in the serum. Deproteinization removes most of thes...
متن کاملDevelopment of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify chikungunya virus.
BACKGROUND The chikungunya virus (CHIKV; Alphavirus, Togaviridae) has emerged in the south Western Indian Ocean since early 2005. A major outbreak of CHIKV infection occurred in Réunion Island, where the virus is transmitted by Aedes albopictus mosquitoes. Facing an outbreak of unprecedented magnitude, we developed a rapid, sensitive, and reliable assay for the detection and quantification of C...
متن کاملDevelopment and application of a most-probable-number-pcr assay to quantify flagellate populations in soil samples.
This paper reports on the first successful molecular detection and quantification of soil protozoa. Quantification of heterotrophic flagellates and naked amoebae in soil has traditionally relied on dilution culturing techniques, followed by most-probable-number (MPN) calculations. Such methods are biased by differences in the culturability of soil protozoa and are unable to quantify specific ta...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2021
ISSN: 0022-1317,1465-2099
DOI: 10.1099/jgv.0.001373